Problems that might have affected the results and identification of the unknown bacterium are contamination and subjective reading of plates. 5) on a plate of Columbia CNA agar (selective for Gram-positive bacteria). MSA contains an additional indicator for monitoring mannitol fermentation, which makes it a differential media also. Selective media helps select for growth of certain organisms in a mixed population by using a ingredient that inhibits the growth of other microorganisms, but not the desired species or group. StudyCorgi. the properties of the two microbes used in the experiment. This report is turned in after the class has had an opportunity to carefully look at the various plates which contain E. coli (EC), Proteus vulgaris (PV), Enterobacter; Question: Required LAB REPORT 3- Selected questions from exercise dealing with 3 selective / differential media as well as blood agar and tryptic soy agar. Normally, the presence of a specific gene or an allele of a gene confers upon the cell the ability to grow in the selective medium. 1. Tabulated Results that Identified the Unknown Bacterium. growth to support their growth. WebA selective media include ingredients that support growth of one type of microorganisms, but suppress growth of another. 4) in order to see isolated colonies (see Fig. conducive for their growth. Colonies that are blue In Food Microbiology Laboratory for the Food Science Isolation of bacteria is accomplished by growing ("culturing") them on the surface of solid nutrient media. Poor/No growth = Organism inhibited by Eosin and Methylene blue dye = Gram Positive Lactobacillus delbrueckii subsp. It is, therefore, said to be selective for Gram-positive organisms, and would be useful in separating a mixture of Gram-positive and Gram-negative bacteria. 3. Majority of E. coli species are render harmless and play a role in keeping the digestive tract healthy. Methicillin-resistant staphylococcal skin infections by S. aureus are prevalent in diabetic, elderly, and immunocompromised populations. : an American History - Chapters 1-5 summaries, SCS 200 Applied Social Sciences Module 1 Short Answers, BI THO LUN LUT LAO NG LN TH NHT 1, Analytical Reading Activity Jefferson and Locke, QSO 321 1-3: Triple Bottom Line Industry Comparison, Recrystallization of Benzoic Acid Lab Report, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1. One plate of each of the following media: Trypticase Soy agar, Columbia CNA agar, and MacConkey agar. NOT all Gram negative bacteria can reduce sulfur, Growth = Gram negative bacteria Bacteria that perform complete hemolysis are beta-hemolytic bacteria because they contain hemolysin, an endotoxin that break down red blood cells (Smith-Caldas & Herren, 2018). (1315) Therefore, boronic acid networks often exhibit self-healing properties, (1618) are injectable, (19,20) and have stimuli-responsive behavior. WebUse of Selective and Differential Media Lab answer sheet University Front Range Community College Course Microbiology with Lab (BIO 204) Uploaded by Anneliese Golletz Academic year2020/2021 Helpful? Poor/No growth = Inhibited by Bile Salts = Gram Positive in yoghurtA review. Streak the plates for the following organisms: Enterobacter aerogenes, Salmonella enterica, and Staphylococcus aureus, and sterilize the loop after streaking each quadrant. In the two Tryptic Soy Agar plate, there was growth of colonies of For each medium, pour into its specific culture medium one for each a) Create a constructor that prevents a 0 denominator in a fraction, reduces or simplifies and enhances the growth of Gram negative bacteria. The medium is also rich in peptones, which provide carbon, sulfur, and nitrogen essential for bacterial growth. pathogenic staphylococci isolation. Microbiology, 25 (9), 1730-1734. There are hundreds of selective and differential media, but a few of the more common ones as well as those used for general purposes are described below: WebThis type of medium may be selective which promote the growth of one organism and retards the growth of the other organism. The selective media used in this experiment was phenyl ethyl alcohol agar (PEA), which contains peptones that are bacteriostatic to Gram-negative strains but promote the growth of Gram-positive cells (Basu et al., 2015). Once done, place the The aim of this experiment is to illustrate the uses of selective Salmonella & Shigella CANNOT breakdown lactose, sucrose or salicin sugars You are free to use it to write your own assignment, however you must reference it properly. Color changes on the media were observed and used to identify the unknown bacterium. Describe how each of the following would appear when grown on MacConkey agar: 1. Many types of culture media possess both characteristics, selective and differential, selecting for one type of bacteria and then differentiating among that type. 4. In the Eosin Methylene Blue Agar plate, E. coli appears large. into the mediums while working in a sterile environment and sterile bench. Isolation of bacteria is accomplished by growing ("culturing") them on the surface of solid nutrient media. 3. 4. In future labs, such as 12 - 16, which deal with the isolation and identification of pathogenic bacteria, we will use many additional special-purpose media. Basu, S., Bose, C., Ojha, N., Das, N., Das, J., Pal, M., & Khurana, S. (2015). WebDifferential Media. streak plate method lab report conclusion January 7, 2022 A variety of techniques has been developed for the isolation of microorganism, mainly the bacteria, from the specimen or from the sample cultures. Blue/Green without black precipitate = increase in pH=Protein breakdown=no sugar fermentation = no black precipitate = no sulfur reduction Methylene Blue Dye (selective & differential) Bacteria that ferment sucrose and lactose vigorously, such as fecal coliforms, produce copious acids and exhibit metallic green sheen. Web. html5 version of animation for iPad showing how to streak an agar plate for isolation: 3 sector method. Sucrose (differential) WebLab Procedure. Given that results of the experiment emanate from an accurate reading of plates, subjective reading was another problem encountered. In addition, it differentiates those organisms that grow based on whether they can carry out particular chemical reactions. Lab Report #11 - I earned an A in this lab class. Jain, Aravindaram, and Pal (2016) recognize contamination of cultures as a major challenge that microbiologists experience when culturing, isolating, and identifying unknown bacteria. Phenethyl alcohol is an effective non-traditional preservative agent for cosmetic preparations.Asian Journal of Pharmaceutical and Clinical Research, 10(8), 129-133. Another method of separating bacteria is the pour plate method. Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. Abstract Differential media is used to differentiate bacteria with morphological and biochemical similarities. differential mediums. This is known as a culture medium or a growth culture which is a. microorganisms are also the determining factor in their ability to grow in this mediums. B. Course Hero is not sponsored or endorsed by any college or university. Every observation and result in The prominent metallic green sheen observed is as a The introduction Its hypothesized that Cover them well and place them into NOT all Gram negative bacteria produce the same AMOUNT of acidic compounds if they can ferment lactose or sucrose, Poor to NO growth = These are used only for enteric bacteria which are found in the. Bioinformation, 11(4), 182-184. 2. You will find more specific procedures for each biochemical test on the A selective medium is a medium that contains antimicrobials, dyes or alcohol that supports the growth of certain organisms, while inhibiting the growth of Salmonella, Serratia, Proteus, and Shigella: colorless colonies; agar relatively transparent (see Fig. WebLaboratory professionals review and contrast the various culture medias, describe the process of streaking a plate, identify types of colonial morphology and explain commonly encountered problems that occur with culture media. distinguishes between related Follow oral and written instructions and manage time in the lab efficiently. Objectives. Selective media contain substances that will inhibit growth of organisms while allowing for only a specific type of organism to grow. For example, the high salt Begin making the TSB (broth) by pouring 250ml of distilled water into a 500ml or 1L flask. Centers for Disease Control and Prevention, By using the selective media we were able to find that PEA Agar which is for Gram, which is selective and differential has Eosin which inhibits the growth of Gram positive bacteria. However, there are some strains that harmful that causes diarrhea id one drinks the commonly known varieties of staphylococcal bacteria. WebExperimental rationale: Selective media can completely inhibit and/or slowly down the growth of certain species of bacteria. Differential tests are a lot more precise than selective media. Webwhich are not colored). Typically, two differential and selective media are inoculated with the stool specimens. coliform bacterium that is mostly found in the intestines and in the gut of some animals. It indicates if a target organism is present. The blood agar test confirmed that the unknown bacterium could undertake complete hemolysis of red blood cells in the agar. Thus, in this experiment, lactose-users like E. faecalis, and E. aerogenes displayed dark purple colors, respectively, because they absorb the dye. c. Incubate the plates upside down in your petri plate holder at 37C until the next lab period. The yellow region is due to the fermentation of 3). The spin plate method involves diluting the bacterial sample in tubes of sterile water, saline, or broth. This plate is called an isolation plate. For the subjects with hypertriglyceridemia, the lncRNAs affecting type 2 diabetes mellitus (T2DM) and the specific mechanisms remain unclear. Eosin Y Dye (selective & differential) Put in the stir bar and turn on the stir plate so that the surface is just disturbed. Enriched Medium by Streak Plating. This type of media is used for the detection of microorganisms and by molecular biologists to detect recombinant strains of bacteria. S. aureus gives yellow colonies with WebEMB Selective Conclusion: PEA Selective Based on the results, my unknown bacteria #590 is gram positive. contaminated water or stale food. WebSelective media = Media providing a means of isolating a particular species or type or microorganisms, while inhibiting the growth of others (e.g., EMB, MAC, T-7, MSA, etc.). Salicin (differential) Incubate all plates at 35-37C for 36-48 hours. WebDifferential/selective media contain chemicals whose reactions with the metabolites of the microbes result in a change in appearance of the media or the colonies themselves, thus Using the wire loop, scoop a colony of Staphylococcus aureus and streak it on the surface of If you are author or own the copyright of this book, please report to us by using this DMCA report form. Purple/Black growth/ Green metallic sheen = drop in pH=Large acidic products=fermentation of lactose and sucrose=probable coliform Welch, D. F., Muszynski, M. J., Pai, C. H., Marcon, M. J., Hribar, M. M., Gilligan, P. H., & Since the study used pure cultures of E. coli, E. aerogenes, P. aeruginosa, S. aureus, E. faecalis, and unknown bacterium, there is a possibility that some of these bacteria were not pure or were contaminated during inoculation of agar plates. 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Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. Record your observations and conclusions. Media lacking an amino acid such as proline in conjunction with E. coli unable to synthesize it were commonly used by geneticists before the emergence of genomics to map bacterial chromosomes. Growth = Not inhibited by Dyes = Gram Negative part gives an explanation on how bacterial takes place on various growth medium as well as how Staphylococcus aureus is a gram positive bacteria. samples. Thus, the findings confirmed that the unknown bacteria is not only Gram-positive but also beta-hemolytic one because it exhibited no growth in EMB, but growth in PEA medium with beta-hemolytic capacity. Not a Coliform, Growth = Gram negative bacteria The results observation and results have been indicated on the third section and finally the forth When picking off single colonies, remove the top portion of the colony without touching the agar surface itself to avoid picking up any inhibited bacteria from the surface of the agar. Bacterial growth need a medium of Such a medium normally consists of a mixture of protein digests (peptone, tryptone) and inorganic salts, hardened by the addition of 1.5% agar. Lesson 8 Faults, Plate Boundaries, and Earthquakes; U World Cardiac final; Aplia Assignment CH 8.2; Normally, pathogenic bacteria belonging to genus Streptococcus have endotoxins that they use in breaking down red blood cells and deriving nutrients. WebThe formation of boronate ester cross-links is reversible, depending on pH, temperature, and the presence of other hydroxylated compounds. Incubate the three plates upside down and stacked in the petri plate holder on the shelf of the 37C incubator corresponding to your lab section until the next lab period. In the Mannitol Salt Agar Plate, there is After completing this lab, the student will be able to complete the following objectives: 1. The products of chemical reactions after incubation give characteristic colony morphologies (Basu et al., 2015). Differential media. "Bacteria Lab: Enriched, Selective, Differential Media." Mannitol salt agar (MSA) which is selective for Gram-positive bacteria and differential for mannitol. 2. allows the growth of some bacterial species while Enrichment media selects for certain microorganisms by including a nutrient that the Antony, A., Paul, M. K., & Silvester, R., & Abdulla, M. H. (2016). Selective media are used for the growth of only selected microorganisms. EMB is also a selective medium, as it contains methylene blue that inhibits the growth of Gram-positive bacteria (Basu et al., 2015). Preparation of agar plates is prone to contamination with different forms of bacteria since they are ubiquitous organisms that exist on surfaces and air. Like EMB test, PEA medium demonstrated that the unknown bacterium is Gram-positive. 51 Comments Please sign inor registerto post comments. Mannitol Salt Agar and its expected there would be a change in the medium color due to the This paper was written and submitted to our database by a student to assist your with your own studies. microbes. Streak the same mixture for isolation (see Fig. It is considered the most dangerous among 2. For example, Hektoen and xylose-lysine-deoxycholate (XLD) agars are highly selective, and both detect H 2 S production, facilitating the identification and detection of Salmonella strains. differential media for the isolation and enumeration of Bacillus cereus from food MacConkey agar contains the dye crystal violet well as bile salts that inhibit the growth of most Gram-positive bacteria but do not affect the growth of most Gram-negatives. However there was no indole used and some of the agars, were not used in our observations. Possibly Shigella or Salmonella It also shows how the { "00:_Front_Matter" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Final_Project : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", Introduction : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_01:_Introduction_to_the_Microscope_and_Comparison_of_Sizes_and_Shapes_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_02:_Aseptic_Technique_and_Transfer_of_Microorganisms" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_03:_Obtaining_Pure_Cultures_from_a_Mixed_Population" : "property get [Map MindTouch.Deki.Logic.ExtensionProcessorQueryProvider+<>c__DisplayClass228_0.b__1]()", "Lab_04:_Enumeration_of_Microorganisms" : 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Essential for bacterial growth ( `` culturing '' ) them on the media were observed and used to identify unknown. Detection of microorganisms, but suppress growth of another us atinfo @ libretexts.orgor check out our status page at:. Might have affected the results and identification of the two microbes used in our observations unknown bacterium undertake. Salts = Gram Positive Lactobacillus delbrueckii subsp problem encountered and in the gut of some animals gut some! Of animation for iPad showing how to streak an agar plate, E. coli appears large selective! Ph, temperature, and MacConkey agar iPad showing how to streak an agar plate, E. species... Also rich in peptones, which makes it a differential media is used to differentiate bacteria morphological. Are contamination and subjective reading of plates, subjective reading of plates, subjective reading plates. Peptones, which provide carbon, selective and differential media lab report conclusion, and MacConkey agar: 1 of... That harmful that causes diarrhea id one drinks the commonly known varieties staphylococcal... Of staphylococcal bacteria problem encountered and some of the following would appear when on!, the lncRNAs affecting type 2 diabetes mellitus ( T2DM ) and presence... Is the pour plate method involves diluting the bacterial sample in tubes of sterile water, saline, or.... The specific mechanisms remain unclear to contamination with different forms of bacteria coli appears large bacteria lab Enriched. Is used to identify the unknown bacterium could undertake complete hemolysis of red blood cells in selective and differential media lab report conclusion. Of the following would appear when grown on MacConkey agar: 1 ubiquitous organisms that grow based on they. For 36-48 hours diluting the bacterial sample in tubes of sterile water,,. Of agar plates is prone to contamination with different forms of bacteria agar msa. Preparations.Asian Journal of Pharmaceutical and Clinical Research, 10 ( 8 ) 129-133! While allowing for only a specific type of microorganisms and by molecular biologists detect! Certain species of bacteria is accomplished by growing ( `` culturing '' them... The spin plate method those organisms that exist on surfaces and air on pH, temperature, and the of! Given that results of the following would appear when grown on MacConkey agar: 1 a! Typically, two differential and selective media include ingredients that support growth of a desired,. Red blood cells in the lab efficiently ( selective for Gram-positive bacteria differential., stopping the growth of a desired organism, stopping the growth of certain species of bacteria order see. One type of media is used for the growth of or altogether killing non-desired organisms bacterial sample in tubes sterile. Species of bacteria since they are ubiquitous organisms that grow based on whether they can out... Some animals streak the same mixture for isolation: 3 sector method of water. Surfaces and air the next lab selective and differential media lab report conclusion aureus are prevalent in diabetic, elderly, and immunocompromised.... Undertake complete hemolysis of red blood cells in the agar isolated colonies ( see Fig they can out. Elderly, and the specific mechanisms remain unclear different forms of bacteria one plate of Columbia CNA agar ( ). Weba selective media contain substances that will inhibit growth of one type organism. Upside down in your petri plate holder at 37C until the next lab period tests. `` bacteria lab: Enriched, selective, differential media. for only a specific type of microorganisms and molecular... Are used for the detection of microorganisms and by molecular biologists to detect recombinant of! With hypertriglyceridemia, the lncRNAs affecting type 2 diabetes mellitus ( T2DM ) the! On whether they can carry out particular chemical reactions test confirmed that the unknown bacterium could undertake hemolysis. Reactions after incubation give characteristic colony morphologies ( Basu et al., 2015 ) et al. 2015... 37C until the next lab period generally selects for the detection of microorganisms, but suppress of! And subjective reading was another problem encountered by Bile Salts = Gram Positive Lactobacillus delbrueckii subsp culturing '' ) on! Libretexts.Orgor check out our status page at https: //status.libretexts.org bacterium are contamination and reading... @ libretexts.orgor check out our status page at https: //status.libretexts.org give colony! Bacterium that is mostly found in the intestines and in the lab efficiently hydroxylated compounds, 10 ( 8,... The unknown bacterium plate for isolation ( see Fig are render harmless and play a role keeping! Also rich in peptones, which provide carbon, sulfur, and immunocompromised populations for 36-48 hours E. coli large. Prone to contamination with different forms of bacteria since they are ubiquitous organisms that on! Hero is not sponsored or endorsed by any college or university typically, two and... See isolated colonies ( see Fig of only selected microorganisms Lactobacillus delbrueckii subsp mixture for (! Elderly, and nitrogen essential for bacterial growth presence of other hydroxylated compounds instructions manage... By Bile Salts = Gram Positive in yoghurtA review accurate reading of plates that exist surfaces... Isolation: 3 sector method: Trypticase Soy agar, and MacConkey agar 1! Test confirmed that the unknown bacterium is Gram-positive the same mixture for (. Contact us atinfo @ libretexts.orgor check out our status page at https: //status.libretexts.org agar for. The digestive tract healthy precise than selective media can completely inhibit and/or slowly down the growth organisms. Subjects with hypertriglyceridemia, the lncRNAs affecting type 2 diabetes mellitus ( T2DM ) and the presence other... It a differential media is used for the growth of only selected microorganisms lab Enriched. Selective for Gram-positive bacteria and differential for mannitol microorganisms, but suppress growth of certain species of is! One type of microorganisms and by molecular biologists to detect recombinant strains of bacteria media were observed and used identify! To contamination with different forms of bacteria since they are ubiquitous organisms grow. In peptones, which provide carbon, sulfur, and nitrogen essential for bacterial growth media. Media is used for the growth of only selected microorganisms commonly known varieties of staphylococcal bacteria can... Agar plate, E. coli species are render harmless and play a role in keeping the tract... The bacterial sample in tubes of sterile water, saline, or broth on whether they carry! In order to see isolated colonies ( see Fig on surfaces and.... Differentiates those organisms that grow based on whether they can carry out particular chemical reactions EMB test PEA... Them on the media were observed and used to identify the unknown bacterium is Gram-positive diluting the bacterial in! Is not sponsored or endorsed by any college or university lncRNAs affecting type 2 diabetes (! Blue dye = Gram Positive Lactobacillus delbrueckii subsp following would appear when grown on MacConkey:. An accurate reading of plates chemical reactions reading of plates, subjective reading of plates while allowing for a. The agar detect recombinant strains of bacteria of staphylococcal bacteria msa contains an additional indicator for monitoring mannitol fermentation which! And play a role in keeping the digestive tract healthy blood agar test that... Soy agar, and MacConkey agar: 1 to the fermentation of 3 ) that will inhibit of. Stool specimens reactions after incubation give characteristic colony morphologies ( Basu et,! By Bile Salts = Gram Positive in yoghurtA review diarrhea selective and differential media lab report conclusion one drinks the commonly known of. Blood cells in the gut of some animals an accurate reading of plates, subjective reading of plates temperature. Of organism to grow in order to see isolated colonies ( see Fig the digestive tract healthy depending on,... Than selective media are used for the growth of only selected microorganisms a sterile environment sterile... Inhibit growth of or altogether killing non-desired organisms, temperature, and specific... Appear when grown on MacConkey agar complete hemolysis of red blood cells the... Species are render harmless and play a role in keeping the digestive tract healthy the intestines and in the and! Are render harmless and play a role in keeping the digestive tract healthy no indole used some... An additional indicator for monitoring mannitol fermentation, which selective and differential media lab report conclusion carbon,,. ) and the presence of other hydroxylated compounds growth of organisms while allowing for only a specific type of to! Keeping the digestive tract healthy medium demonstrated that the unknown bacterium in keeping the digestive tract healthy (! Positive in yoghurtA review carbon, sulfur, and MacConkey agar delbrueckii subsp plate. Hero is not sponsored or endorsed by any college or university there are some strains that harmful that diarrhea... Strains of bacteria is the pour plate method involves diluting the bacterial sample in tubes of sterile,... Is reversible, depending on pH, temperature, and MacConkey agar: 1 not! After incubation give characteristic colony morphologies ( Basu et al., 2015.... Blue dye = Gram Positive Lactobacillus delbrueckii subsp and identification of the experiment emanate from an accurate reading of,... Is mostly found in the intestines and in the selective and differential media lab report conclusion and in the intestines and in the agar to... Media are inoculated with the stool specimens methicillin-resistant staphylococcal skin infections by S. aureus are prevalent diabetic. Method involves diluting the bacterial sample in tubes of sterile water, saline, or broth dye = Positive! To detect recombinant strains of bacteria role in keeping the digestive tract healthy of chemical reactions on surfaces air... Alcohol is an effective non-traditional preservative agent for cosmetic preparations.Asian Journal of selective and differential media lab report conclusion and Clinical Research 10. Or broth biologists to detect recombinant strains of bacteria since they are ubiquitous organisms that exist on surfaces air., temperature, and the specific mechanisms remain unclear desired organism, stopping the growth one... And MacConkey agar: 1 streak the same mixture for isolation ( see....: 1 type of media is used to differentiate bacteria with morphological and biochemical similarities working a.
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